An outlook with a discussion of the current limitations to design screening devices for membrane proteins is in Chapter 7.Future developments of the work presented in this thesis include: addressing the switch on/off of individual pores for controlled transport across selected pores; analyzing the transport of ions and low-molecular-weight drug candidates per single nanopore; and test the universal applicability of the designed assay platforms by integrating different types of membrane proteins.
It was also demonstrated that the protein density could be varied in a wide range without impairing the formation of the lipid bilayer.
Although improvements are needed for the electrochemical measurements of membrane protein activity, yet the potential application of the integrated platform for ion channel protein assays is demonstrated.
Several properties of the smart PMAA brush contributed to the functionality of such protein assays.
Controlled transport of ions and molecules through nanopores, support of pore-spanning artificial lipid bilayers and positioning of membrane proteins over the pores were achieved with the explored PMAA-brush functionalized platforms.
The formation of the suspended artificial lipid bilayers was achieved by spontaneous liposome rupture and fusion.
Thesis Transport Aids Essay Conclusion
Enhanced membrane resistance as determined by electrochemical impedance spectroscopy (EIS) and free diffusion of dyed lipids observed as fluorescence recovery after photobleaching (FRAP) confirmed the presence of the lipid bilayer.All the results presented in this chapter rationalize the use of nanoporous platforms functionalized with PMAA brushes for controlled gating applications, for example as electrochemical (bio)sensors or nanoscale valves.In Chapter 6, a description is provided discussing functionalization of silicon-based nanopore array chips functionalized with PMAA brush, which were used as supports for pore-spanning lipid bilayers.The shift is due to ion confinement effects in the dense brush, and brings the conformational switching of PMAA brushes closer to physiological conditions.This is relevant for biologically oriented applications in miniaturized devices with rapid response times.The functionalized pores had higher electrical resistance when the brush was in a neutral state and lower resistance when the brush was charged.The results of these measurements, combined with diffusion experiments using a fluorescent dye to investigate mechanical gating, demonstrated the controlled transport of ions and small molecules across the pores.As additional review of the literature background for the work presented in this thesis, an overview on pore-spanning lipid bilayers for functional assays of membrane proteins is presented in Chapter 3.The role of membrane proteins as drug targets is introduced and the current limitations in the development of such assays are discussed.Students have to prepare the draft of the thesis by the fourth week of the semester.Based on this draft the Consultant composes the specification sheet of thesis, which contains the final title, 3-5 bullet points about the content, name of the External Supervisor and Final Examination subjects.
Comments Thesis Transport
Unidimensional and Evolution Methods for Optimal.
H thesis. Thèse de doctorat • Tesi di perfezionamento. Unidimensional and Evolution. transport map that seems close enough to the optimal map for most.…
Transport of Trapped-Ion Qubits within a Scalable. - NIST
The final copy of this thesis has been examined by the signatories, and we find that. of the ions during transport can negatively impact computational fidelity.…
HKU PhD graduate wins best PhD thesis on urban mobility in.
PhD thesis on urban mobility in cities of developing countries with his thesis 'Transportation Disadvantage and Social Exclusion in Pakistan'.…
Ph. D. thesis of Wilma de Groot - Prof. GJ Vancso
Wilma de Groot thesis cover Smart polymer brushes in nanopores. Controlled transport of ions and molecules through nanopores, support of pore-spanning.…
Challenging the Derived Transport-Demand Thesis.
One of the core concepts in transport and economic geography states that transportation is a derived demand, both for passengers and for freight transport alike.…
Dissertation Thesis Topics CEITEC - výzkumné centrum
The disserations thesis topics proposed within the Research programme. Transport and Magnetic Properties, Functional Properties of Nanostructures, Plasma.…
Master thesis Increasing customer satisfaction with public.
You are reading my master thesis for the study Transport. Customer satisfaction with public transport is defined as the degree to which an.…
University of Groningen Magnon spin transport in magnetic.
The work described in this thesis was performed in the research group. magnon spin transport measurement technique we developed in this thesis promises.…
TML Thesis Prize - Transport & Mobility Leuven
Are you about to accomplish a thesis on mobility or traffic and transport, safety, traffic and spatial planning, transport technology or freight transport at a Belgian.…