Thesis On Isolation Of Bacteria

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Optimization of growth condition Three semi-solid media as NA (Nutrient Agar), BCDA (czapek dox agar medium (Basic)) and ACDA (Czapek Dox Agar medium (Acidic)) were used to optimize the cultural media of isolated bacteria.

The p H were adjusted to 5.2, 6.5, 7.2, 8.9 and 10.2 in NA medium; 2.2, 3.2, 4.0, 5.5 and 6.9 in BCDA medium; and 7.1, 7.6, 9.1, 10.06 and 12.10 in ACDA medium.

Sample (soil mixed with waste) was collected in sterile zip-lock plastic maintaining aseptic conditions, stored at 4 °C and marked accordingly to their source and location.

The collected samples were brought to the laboratory for isolation of soil bacteria and the moisture content and p H of sample were documented.

With the doubling of population and changing lifestyle pattern of the inhabitants the quantity of municipal waste generated is increasing in an alarming rate.

Most of this waste is subjected to dumping in a specified disposal yard.

The samples were weighed several times until a constant weight was achieved.

Samples moisture content was calculated employing the following formula provided by AWPA.100 Where, MC is moisture content, W is the original weight and w is the constant weight after oven drying.

Study area Soil sample was collected from waste disposal site of Barrackpore Municipality in Barrackpore (latitude 22°44’N and longitude 88°30’E) in North 24 Parganas District of West Bengal State in Eastern India and from Dhapa situated at 88°20’E-88°35’E and 20°25’N-20°35’N at Kolkata District in West Bengal.

Collection of samples A total of 10 waste samples were collected 5 from waste disposal site of Barrackpore Municipality and rest 5 from Dhapa, landfill site of city of Kolkata.

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