The group of Davis utilized the olefin metathesis reaction to perform post-expressional protein modifications [10-12].For example, a single cysteine mutant of subtilisin from Bacilus lentus (SBL-S156C) was modified via sulfide bond formation with allyl cysteine displaying an allyl function on the protein surface.
The group of Davis utilized the olefin metathesis reaction to perform post-expressional protein modifications [10-12].For example, a single cysteine mutant of subtilisin from Bacilus lentus (SBL-S156C) was modified via sulfide bond formation with allyl cysteine displaying an allyl function on the protein surface.Tags: Steps For A Research PaperStudying Abroad Essay ConclusionValue Of Discipline In Student Life Short EssayArgumentative Essay On Music CensorshipLord Of The Rings Essay HelpAssignment On
The combination of an engineered protein with a synthetic metal catalyst leads to artificial metalloproteins [20-23].
In the case of a metathesis catalyst, so-called artificial metatheases are obtained, which could open new areas of biological applications .
Further applications would be the implementation of olefin metathesis into natural metabolic pathways to allow synthesis of fine chemicals .
Also, a targeted reaction in a certain environment within a living cell with a precise release or activation of the catalyst would enable new ways of drug delivery.
These catalysts do not only show stability towards moisture, but can also be directly used in water, allowing to perform olefin metathesis reactions in aqueous solutions [8,9].
Olefin metathesis is not known in biological systems and therefore can be regarded as bio-orthogonal.
The protein as second coordination sphere might take influence on the formation of the metallacyclobutane that was initially postulated by Chauvin .
The formation of the E or the Z product is dependent on the orientation of the R groups in this step of the catalytic cycle (Scheme 1).
One of the challenges to overcome in the construction of artificial metalloproteins is to find a method to incorporate a synthetic metal complex into a protein scaffold . In Figure 1, the three commonly utilized methods to incorporate a synthetic cofactor are shown.
Strategies utilized are supramolecular, dative and covalent anchoring.