Daniel Wagenaar Thesis

Daniel Wagenaar Thesis-69
Burst control could not be achieved with single electrode stimulation.For the final chapter, I tested various protocols for inducing plasticity by tetanic stimulation.The Wagenaar Lab is part of the Division of Biology and Biological Engineering at the California Institute of Technology.

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He was always completely uninhibited in both his criticism and his praise, and while it hurt when he dismissed a draft for a paper as utterly obscure, he was usually right.Update (August 2019): Both positions have been filled.(Regardless, exceptional self-funded candidates will still be considered.) We are looking for up to two postdoctoral scholars to work on reconstructing the connectome of leech ganglia from which we have recorded the activity of all the neurons during a variety of behaviors.Armed with his many suggestions, I would then rewrite the thing, until he gave it his sign of approval, “it reads like a novel.” Jerry always demanded absolute scientific integrity.If you tried to make a claim based on dubious data, he’d say, “that is nonsense, and you know it. Let me begin by thanking Jerry Pine and Steve Potter, the best advisers I could have wished for.From the moment I first stepped into Jerry’s lab, when I visited Caltech as a prospective, I knew it would be a good place to work.I designed and built hardware to deliver electrical stimuli in arbitrary patterns, developed software to remove stimulation artifacts from recordings, and studied the efficacy of several voltage-defined and current-defined stimulus waveforms. Thus, stimuli can be made dependent on a culture's activity with only 15 ms lag-time.We hypothesized that synchronized bursting can dominate activity patterns, because lack of external input puts cultures in a hypersensitive state.Past funding came from the Burroughs Wellcome Fund in the form of a Career Award at the Scientific Interface, from the University of Cincinnati and from The Broad Foundations.All support, past and present, is gratefully acknowledged.


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